Qualitative and quantitative assessment of platelet rich plasma (PRP) gel as a local delivery system of antibiotics. In vitro Study
DOI:
https://doi.org/10.1016/j.rccot.2016.03.003Keywords:
platelet rich plasma gel, antibiotics local delivery system, muscle skeletal infectionAbstract
Introduction: It has been theorized that antibiotic loaded autologous platelet rich plasma (PRP) gel inhibits in vitro growth of bacteria, resembling an antibiotic local delivery system.
Materials & methods: Fresh PRP obtained from healthy donors was activated with CaCl2 and loaded with 12 different antibiotics (oxacillin, gentamicin, vancomycin, trimethoprim/sulfamethoxazole, clindamycin, erythromycin, linezolid, tigecycline, tetracycline, chloramphenicol, rifampicin, and ciprofloxacin). The ability of the PRP to retain and release antibiotics was evaluated by conventional S. aureus (ATCC 29213) bacterial growth inhibition through the Kirby Bauer method at several times (1 to 21 hours). Initial and final antibiotic load of each PRP sample was evaluated by high-pressure liquid chromatography (HPLC).
Results: We had found out that rifampicin, tetracycline, vancomycin, tigecycline, clindamycin, and trimethoprim/sulfamethoxazole, showed a slow release rate (more than 21 hours), as well as gentamicin, erythromycin, ciprofloxacin, linezolid, oxacillin and chloramphenicol showed a fast release rate (less than 21 hours), all of them producing effectively bacterial growth inhibition. Vancomycin, tetracycline, trimethoprim/sulfamethoxazole, erythromycin, ciprofloxacin, linezolid, and chloramphenicol required a higher concentration (more than 4 mg/ml) on PRP gel to effectively achieve in vitro bacterial growth inhibition. PRP showed an ability to retain 12.5% of oxacillin at 21 hours.
Discussion: PRP gel showed a good retention and release ability with all the evaluated antibiotics.
Evidence Level: III.
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